Summary
Type: SPE
Application #: 304170
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning: 2 mL methanol, then 2 mL 10 mmol/L ammonium acetate, pH 6.5 (Do not let run the column dry.)
Sample aspiration: 2 mL of pretreated sample
Washing: 2 mL water – acetonitrile (9:1, v/v)
Elution: 2 mL methanol – acetonitrile (1:1, v/v)
Evaporation: to dryness (40 °C, light stream of nitrogen)
Reconstitution: 2 mL water + 5 mmol/L HFBA – acetonitrile (9:1, v/v), pH 2.3
Filtered sample (0.2 µm nylon filter, e.g., CHROMAFIL® PA) was transferred directly into an HPLC vial.
Authors: Mohamed, R. et al.
Source: J. Chromatogr. A 1114 (2006) 62–72